{"id":305313,"date":"2024-10-19T20:53:38","date_gmt":"2024-10-19T20:53:38","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-164182014\/"},"modified":"2024-10-25T18:28:10","modified_gmt":"2024-10-25T18:28:10","slug":"bs-en-164182014","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-164182014\/","title":{"rendered":"BS EN 16418:2014"},"content":{"rendered":"

This European Standard specifies a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.<\/p>\n

Compared to the EN 15845[1]<\/sup>, HepG2 cells are more representative of a human oral exposure to xenobiotics, due to the presence in the cells of phase I, II and III enzymes of the metabolism.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nContents Page <\/td>\n<\/tr>\n
5<\/td>\nForeword <\/td>\n<\/tr>\n
6<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
7<\/td>\n4 Principle
5 Reagents
5.1 Liquid scintillant, for tritium counts on dry filters
5.2 Culture media
5.2.1 Culture media \u2013 quality and storage
5.2.2 Medium for routine culture <\/td>\n<\/tr>\n
8<\/td>\n5.2.3 Concentrated culture medium for testing samples
5.3 Solution for rinsing cell lawns
5.4 Cell dissociation reagent <\/td>\n<\/tr>\n
9<\/td>\n5.5 Trypan blue, at 0,4 % (w\/V)1)
5.6 Dimethyl sulfoxide (DMSO), analytical-grade
5.7 Sodium dodecyl sulphate (SDS), for analysis, at 3 % (m\/V)
5.8 Trichloroacetic acid (TCA), for analysis
5.9 Ethanol, 95 % to 96 % (v\/V)
5.10 [5,6-3H] uridine
6 Cell line
6.1 Generating the cell strain
6.2 Maintaining the cell strain <\/td>\n<\/tr>\n
10<\/td>\n6.3 Storing the cell strain
7 Food simulants used for testing
7.1 Reference water (3.1)
8 Cleaning laboratory glassware
8.1 Cleaning liquids for laboratory glassware
8.2 Cleaning procedure for laboratory glassware <\/td>\n<\/tr>\n
11<\/td>\n9 Equipment
9.1 Equipment for the migration test
9.2 Cell culture equipment
9.3 Equipment used for cytotoxicity testing <\/td>\n<\/tr>\n
12<\/td>\n10 Preparation of specimens
10.1 General
10.2 Paper and board intended for wet contact
11 Cytotoxicity assessment
11.1 Principle
11.2 General <\/td>\n<\/tr>\n
13<\/td>\n11.3 Cell seeding
11.4 Preparation of samples
11.5 Cell culture treatment <\/td>\n<\/tr>\n
14<\/td>\n11.6 Preparation of the chromatography sheet
11.7 Kinetics of uridine incorporation in the cell RNA
11.8 Measurements of the RNA synthesis
11.8.1 option A <\/td>\n<\/tr>\n
15<\/td>\n11.8.2 option B
12 Expression of the results
12.1 Graphic representation of the results <\/td>\n<\/tr>\n
16<\/td>\n12.2 Calculation of percentage RNA synthesis and the validity of the test
12.2.1 General
12.2.2 Reference sample
12.2.3 Negative control sample
12.2.4 Positive control sample <\/td>\n<\/tr>\n
17<\/td>\n12.2.5 Test sample
13 Interpretation of the results
13.1 Results for the reference sample
13.2 Results of the positive control sample
13.3 Results for the test sample
14 Precision
Table 1 \u2014 Repeatability and reproducibility found in an interlaboratory test
15 Test report <\/td>\n<\/tr>\n
19<\/td>\nAnnex A (informative) 96-well plates configuration
Table A.1 \u2014 96-well plates configuration <\/td>\n<\/tr>\n
20<\/td>\nAnnex B (informative) RNA Synthesis rate inhibition cytotoxicity test work flow
Table B.1 \u2014 RNA Synthesis rate inhibition cytotoxicity test work flow <\/td>\n<\/tr>\n
21<\/td>\nAnnex C (informative) Validation of the two methods (option A and B)
Table C.1 \u2014 Validation of the two methods (option A and B) <\/td>\n<\/tr>\n
22<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Paper and board. Determination of the cytotoxicity of aqueous extracts using a metabolically competent hepatoma cell line (HepG2)<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2014<\/td>\n24<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":305317,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-305313","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/305313","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/305317"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=305313"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=305313"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=305313"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}