{"id":15218,"date":"2024-10-16T21:49:17","date_gmt":"2024-10-16T21:49:17","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/astm-f2664-2007\/"},"modified":"2024-10-24T12:09:40","modified_gmt":"2024-10-24T12:09:40","slug":"astm-f2664-2007","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/astm\/astm-f2664-2007\/","title":{"rendered":"ASTM-F2664 2007"},"content":{"rendered":"<\/p>\n
1.1 This guide describes protocols that can be used to measure the strength of the adhesive bond that develops between a cell and a surface as well as the force required to detach cells that have adhered to a substrate. Controlling the interactions of mammalian cells with surfaces is fundamental to the development of safe and effective medical products. This guide does not cover methods for characterizing surfaces. The information generated by these methods can be used to obtain quantitative measures of the susceptibility of surfaces to cell attachment as well as measures of the adhesion of cells to a surface. This guide also highlights the importance of cell culture history and influences of cell type.<\/p>\n
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.<\/p>\n
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
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1<\/td>\n | Scope Referenced Documents Terminology <\/td>\n<\/tr>\n | ||||||
2<\/td>\n | Significance and Use Cell Attachment Assays TABLE 1 <\/td>\n<\/tr>\n | ||||||
3<\/td>\n | Measurement of Cell Detachment <\/td>\n<\/tr>\n | ||||||
4<\/td>\n | FIG. 1 FIG. 2 <\/td>\n<\/tr>\n | ||||||
5<\/td>\n | Additional Considerations for Cell Adhesion\/Detachment Assays FIG. 3 <\/td>\n<\/tr>\n | ||||||
6<\/td>\n | X1. SOURCES OF VARIATION IN CELL ADHESION ASSAYS\u2014CELLULAR X1.1 Cell Type, Harvesting and Culture <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | X2. SOURCES OF VARIATION IN CELL ADHESION ASSAYS\u2014BIOMATERIALS X2.1 X2.2 Influences of Surfaces on Cell Adhesion X2.3 Cellular attachment to the extracellular matrix (ECM) <\/td>\n<\/tr>\n | ||||||
8<\/td>\n | X2.4 Scaffolds X2.5 Cell Binding to Biomaterial Surfaces <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" F2664-07 Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods<\/b><\/p>\n |