BSI PD ISO/TS 20787:2017:2018 Edition
$142.49
Nanotechnologies. Aquatic toxicity assessment of manufactured nanomaterials in saltwater lakes using Artemia sp. Nauplii
| Published By | Publication Date | Number of Pages |
| BSI | 2018 | 24 |
This document specifies a test method, aiming to maximize repeatability and reliability of testing, to determine whether MNMs are toxic to aquatic organisms, specifically Artemia sp. nauplius.
This document is intended to be used by ecotoxicological laboratories that are capable in the hatching and culturing of Artemia sp. and the evaluation of toxicity of nanomaterials using Artemia sp. nauplius.
This method uses Artemia sp. nauplii in a simulated environment, artificial seawater, to assess effects of nanomaterials.
This document is applicable to MNMs that consist of nano-objects such as nanoparticles, nanopowders, nanofibres, nanotubes, nanowires, as well as aggregates and agglomerates of such MNMs.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 7 | Foreword |
| 8 | Introduction |
| 9 | 1 Scope 2 Normative references |
| 10 | 3 Terms and definitions |
| 13 | 4 Materials 4.1 Test organism 4.2 Chemicals 5 Technical equipment 5.1 Adequate apparatus for temperature control. 5.2 Microscope. 5.3 Binocular stereoscope. 5.4 Centrifuge. 5.5 Air pump. 5.6 Single channel pipettes. 5.7 Laboratory balance. |
| 14 | 5.8 Laboratory water purification system. 5.9 Laboratory oven. 5.10 Laboratory autoclave. 5.11 Centrifuge. 5.12 Sonication system. 5.13 Hot plate stirrer. 5.14 Oxygen meter. 5.15 Thermometer. 5.16 pH meter. 5.17 Salt meter (salinity meter). 5.18 Multiparameter photometer. 5.19 Light source. 5.20 Adequate apparatus for the control of the lighting regime and measurement of light intensity (lux meter). 5.21 Equipment for the determination of total organic carbon. 5.22 Equipment for the determination of chemical oxygen demand (COD). 6 Preparation and characterization of dispersion of nanomaterial 6.1 Dispersion preparation |
| 15 | 6.2 Dispersion characterization 6.3 Dispersion stability in stock suspension 6.4 Dispersion stability in artificial seawater 6.5 Preparation of exposure media for toxicity tests 7 Hatching procedure 7.1 General 7.2 Dilution water |
| 16 | 7.3 Storage of cysts 7.4 Disinfection of Artemia sp. cysts 7.5 Hatching method of Artemia sp. cysts 7.6 Harvesting of nauplii |
| 17 | 7.7 Calculation of hatching percentage 8 Effect of nanomaterial on Artemia sp. nauplii 8.1 Test groups and controls 8.2 Test concentrations |
| 18 | 8.3 Exposure condition 8.4 Duration 8.5 Observations 8.6 Analytical measurements 9 Data analysis 10 Test report 10.1 Test procedure |
| 19 | 10.2 Information to include in the report 10.2.1 Test nanomaterial 10.2.2 Test species 10.2.3 Test conditions 10.2.4 Bioassay results 11 Results validity |
| 21 | Bibliography |
