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BS ISO 23641:2021

$102.76

Flexible cellular polymeric materials. Determination of antibacterial effectiveness

Published By Publication Date Number of Pages
BSI 2021 22
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This document specifies a method of determining the antibacterial effectiveness of open-cell flexible cellular polymeric antibacterial treated materials, including their intermediate and final products.

This document is suitable for flexible cellular polymeric materials because the test procedure enables the test inoculum to efficiently contact with the surface of open cell in the flexible cellular polymeric materials.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Judgement criteria of antibacterial effectiveness
5 Test methods
5.1 Bacteria to be used for the tests
5.2 Reagents and materials
11 5.3 Equipment and apparatus
12 5.4 Sterilization methods
5.4.1 General
5.4.2 Dry-heat sterilization
5.4.3 High-pressure steam sterilization
13 5.4.4 Flame sterilization
5.5 Medium and buffer
5.5.1 General
5.5.2 1/500 nutrient broth medium (1/500 NB)
5.5.3 Slant culture medium
5.5.4 Standard nutrient agar medium (SA medium)
5.5.5 Phosphate-buffered physiological saline
5.6 Preservation of test strain
15 5.7 Procedure
5.7.1 General
5.7.2 Pre-culture of bacteria
5.7.3 Preparation of test specimens
5.7.4 Sterilization of specimens
5.7.5 Preparation of test inoculum
16 5.7.6 Inoculation onto the test specimens
17 5.7.7 Shaking incubation of the test specimens and the control group
5.7.8 Measurement of viable bacteria cell count
18 5.7.9 Notation of viable bacteria cell count
5.8 Expression of the results
5.8.1 Requirements for test validity
19 5.8.2 Calculation of the antibacterial activity value
6 Test report
21 Annex A (normative) Pour plate culture method
BS ISO 23641:2021
$102.76