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BS ISO 13496:2021

BS ISO 13496:2021

$198.66

Meat and meat products. Detection and determination of colouring agents

Published By Publication Date Number of Pages
BSI 2021 64
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This document specifies a detection method using thin-layer chromatography and a determination method using high performance liquid chromatography (HPLC) for synthetic colouring agents in meat and meat products.

This document specifies the HPLC method as the reference method.

This document is applicable to meat and meat products, including livestock and poultry products.

The method using thin-layer chromatography can detect the following colouring agents:

Synonyms and identity numbers of these colouring agents are listed in Annex A. The plant colours and plant extracts which have been observed not to interfere with this method are listed in B.1. Natural colours which in some cases have been shown to interfere with this method are listed in B.2.

The method using HPLC can detect the following colouring agents:

Chromatograms of these standard reference colours are shown in Annex D.

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 1 Scope
2 Normative references
8 3 Terms and definitions
4 Principle
4.1 Thin-layer chromatography
4.2 HPLC
5 Sampling
6 Preparation of test sample
7 Test method of thin-layer chromatography
7.1 Reagents
10 7.2 Apparatus
7.3 Procedure
11 7.3.1 Test portion
7.3.2 Fatty samples
7.3.3 Non-fatty samples
7.3.4 Transfer of the colours to polyamide powder
12 7.3.5 Elution and concentration of isolated colours
7.3.6 Thin-layer chromatographic separation
7.3.7 Confirmation
8 Test method of HPLC
8.1 Reagents
13 8.2 Apparatus
8.3 Procedure
8.3.1 Test portion
8.3.2 Fatty samples
8.3.3 Non-fatty samples
14 8.3.4 Transfer of the colours to polyamide powder
8.3.5 Elution and concentration of isolated colours
8.3.6 HPLC analysis
15 8.4 Calculation
8.5 Precision
8.6 Limit of detection (LOD) and limit of quantification (LOQ)
9 Test report
16 Annex A (informative) Synonyms and identity numbers of synthetic, water-soluble colouring agents
17 Annex B (informative) Possible interference by colours
18 Annex C (informative) Absorbance spectra
20 Annex D (informative) Chromatogram and wavelength
21 Annex E (informative) Interlaboratory testing
63 Bibliography

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BS ISO 13496:2021
$198.66