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BS EN ISO 9167:2019

BS EN ISO 9167:2019

$167.15

Rapeseed and rapeseed meals. Determination of glucosinolates content. Method using high-performance liquid chromatography

Published By Publication Date Number of Pages
BSI 2019 38
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This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution.

This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out.

NOTE

This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal.

Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification.

The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Reagents
5.5 Internal standard.
11 5.6 Sinigrin solution.
5.7 Glucotropaeolin solution.
12 6 Apparatus
6.3 pH-meter.
13 7 Sampling
14 8 Preparation of the test sample
9 Procedure
9.1 Test portion
9.2 Extraction of glucosinolates
15 9.3 Blank test
9.4 Preparation of ion-exchange columns
9.5 Purification and desulfatation
9.6 Chromatography with gradient elution
9.6.1 General
16 9.6.2 Adjustment of the apparatus
17 10 Expression of results
10.1 Calculation of the content of each glucosinolate
10.2 Relative proportionality factors
18 10.3 Calculation of the total glucosinolate content
11 Precision
11.1 Interlaboratory test
11.2 Repeatability
11.3 Reproducibility
12 Test report
20 Annex A (informative) Results of interlaboratory trials — Gradient elution HPLC method
22 Annex B (normative) Checking of the titre of the prepared internal standard solution
23 Annex C (normative) Preparation and test of purified sulfatase solution and checking of the desulphation step on ion-exchange columns
28 Annex D (informative) HPLC system and column performance criteria qualification
30 Annex E (informative) Elution in the isocratic mode
36 Bibliography

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BS EN ISO 9167:2019
$167.15