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BS EN ISO 23753-1:2019+A1:2020

$102.76

Soil quality. Determination of dehydrogenases activity in soils – Method using triphenyltetrazolium chloride (TTC)

Published By Publication Date Number of Pages
BSI 2020 18
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This document specifies a method for determining the activity of dehydrogenases enzymes in soil using 2,3,5-triphenyltetrazolium chloride (TTC).

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
Anchor 3
Foreword to amendment A1
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Limitations
10 6 Reagents and materials
6.1 Soil.
6.5 Analytical grade ethanol (96 %).
6.6 Triphenylformazan (TPF) solutions.
11 7 Apparatus
7.1 Visible light spectrophotometer.
7.2 pH meter.
7.3 Suitable U-bottom tubes (35 to 50) ml, volumetric flasks, pipettes and cuvettes.
8 Procedure
8.1 Establishment of standard curve
8.2 Sampling
12 9 Calculation
10 Validity criteria
10.1 Standard curve
10.2 Samples
13 11 Test report
14 Annex A (informative) Results of modified parameters
17 Bibliography
BS EN ISO 23753-1:2019+A1:2020
$102.76