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BS EN ISO 21150:2015+A1:2022

BS EN ISO 21150:2015+A1:2022

$142.49

Cosmetics. Microbiology. Detection of Escherichia coli

Published By Publication Date Number of Pages
BSI 2022 28
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PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
5 European foreword
Endorsement notice
6 Blank Page
8 Foreword
9 Introduction
11 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principle
5 Diluents and culture media
5.1 General
13 5.2 Diluent for the bacterial suspension (tryptone sodium chloride solution)
5.2.1 General
5.2.2 Composition
5.2.3 Preparation
5.3 Culture media
5.3.1 General
5.3.2 Agar medium for the suitability test (see Clause 11) [soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA)]
14 5.3.3 Enrichment broth
5.3.4 Selective agar medium for isolation of Escherichia coli
15 Blank Page
16 5.3.5 Selective agar medium for confirmation of Escherichia coli
17 6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension in the enrichment broth
9.2.1 General
18 9.2.2 Water-miscible products
9.2.3 Water-immiscible products
9.2.4 Filterable products
9.3 Incubation of the inoculated enrichment broth
9.4 Detection and identification of Escherichia coli
9.4.1 Isolation
9.4.2 Identification of Escherichia coli
19 10 Expression of the results (detection of Escherichia coli)
11 Neutralization of the antimicrobial properties of the product
11.1 General
11.2 Preparation of inoculum
11.3 Suitability of the detection method
11.3.1 Procedure
20 11.3.2 Interpretation of suitability test results
12 Test report
21 Annex A (informative) Other enrichment broths
24 Annex B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
25 Bibliography

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BS EN ISO 21150:2015+A1:2022
$142.49