BS EN ISO 21149:2017+A1:2022

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Cosmetics. Microbiology. Enumeration and detection of aerobic mesophilic bacteria

Published By	Publication Date	Number of Pages
BSI	2022	40

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Categories: 07.100.40 - Cosmetics microbiology, BSI

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PDF Catalog

PDF Pages	PDF Title
2	undefined
4	European foreword Endorsement notice
5	European foreword Endorsement notice
6	Blank Page
9	Foreword
11	1 Scope 2 Normative references 3 Terms and definitions
12	4 Principle 4.1 General 4.2 Plate count 4.3 Membrane filtration
13	4.4 Detection of bacteria by enrichment 5 Diluents, neutralizers and culture media 5.1 General 5.2 Neutralizing diluents and diluents
14	5.3 Diluent for the bacterial suspension (tryptone sodium chloride solution) 5.4 Culture media
17	Blank Page
18	Blank Page
19	6 Apparatus and glassware 7 Strains of microorganisms 8 Handling of cosmetic products and laboratory samples 9 Procedure 9.1 General recommendation 9.2 Preparation of the initial suspension 9.2.1 General
20	9.2.2 Water-miscible products 9.2.3 Water-immiscible products 9.3 Counting methods 9.3.1 Dilutions for counting methods 9.3.2 Plate-count methods
21	9.4 Enrichment 9.4.1 General 9.4.2 Incubation of the sample 10 Counting of colonies (plate counts and membrane filtration methods) 11 Detection of growth (enrichment method)
22	12 Expression of results 12.1 Method of calculation for plate count
23	12.2 Interpretation 12.3 Examples
25	12.4 Detection after enrichment 13 Neutralization of the antimicrobial properties of the product 13.1 General
26	13.2 Preparation of inoculum 13.3 Suitability of counting methods 13.3.1 Principle 13.3.2 Suitability test of the pour-plate method 13.3.3 Suitability of the surface spread method 13.3.4 Suitability of the membrane filtration method
27	13.4 Suitability of the detection method by enrichment 13.4.1 Procedure 13.4.2 Interpretation of results 13.5 Interpretation of suitability test results
28	14 Test report
29	Annex A (informative) Other neutralizing diluents
31	Annex B (informative) Other diluents
32	Annex C (informative) Other culture media
35	Annex D (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
36	Bibliography