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BS EN ISO 20418-3:2020

BS EN ISO 20418-3:2020

$142.49

Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres – Peptide detection using LC-MS without protein reduction

Published By Publication Date Number of Pages
BSI 2020 32
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This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.

NOTE 1

The composition of non-animal hair fibres can be measured by ISO 1833 (all parts) . Both results are combined to determine the total fibre composition.

The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827[ 4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present.

NOTE 2

In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts) ].

PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
6 Foreword
7 Introduction
8 1 Scope
2 Normative references
3 Terms and definitions
9 4 Symbols and abbreviated terms
5 Principle
10 6 Reagents
7 Apparatus
11 8 Test method
8.1 Sampling
8.2 Preliminary identification
8.3 Wash for degreasing
8.4 Powderization of fibres
8.5 Trypsin digestion
12 8.6 Marker peptides
8.7 LC-MS analysis
8.8 Evaluation of the validity of observed data
8.9 Calculation of correction factor
8.9.1 Correction factor
13 8.9.2 Correction factor for Cas1 against She1 and Yak1 (kc)
8.9.3 Correction factor for Yak2 against She2, She3 and Cas2 (ky)
8.9.4 Correction factor for alpaca and camel (ka)
8.9.5 Correction factor for angora (kr)
8.10 Calculation of blending ratio
8.10.1 Calculation of blending ratio of cashmere, sheep wool and yak
14 8.10.2 Calculation by She1, Cas1, Yak1 and kc
8.10.3 Calculation by She2, She3, Cas2, Yak2 and ky
8.10.4 Calculation by She1, She2, She3, Cas2, Yak1
15 8.10.5 Calculation of blending ratio of camel, alpaca and angora
16 9 Test report
17 Annex A (informative) Marker peptides of cashmere, sheep wool and yak fibres
20 Annex B (informative) Example of LC-MS analysis conditions
23 Annex C (informative) Analysis of camel, alpaca and angora fibres
26 Annex D (informative) Example of marker peptide mass chromatograms
30 Annex E (informative) Results of interlaboratory study
31 Bibliography

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BS EN ISO 20418-3:2020
$142.49