ASTM-E724 2021

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E724-21 Standard Guide for Conducting Static Short-Term Chronic Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs

Published By	Publication Date	Number of Pages
ASTM	2021

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Categories: 07.100.20 - Microbiology of water, ASTM

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Description

ASTM E724-21

Active Standard: Standard Guide for Conducting Static Short-Term Chronic Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs

ASTM E724

Scope

1.1 This guide describes procedures for obtaining laboratory data concerning the acute effects of a test material on embryos and the resulting larvae of four species of saltwater bivalve molluscs (Pacific oyster, Crassostrea gigas Thunberg; eastern oyster, Crassostrea virginica Gmelin; quahog or hard clam, Mercenaria mercenaria Linnaeus; and the mussel species complex (Mytilus spp.) including the blue mussel, Mytilus edulis Linnaeus; the Mediterranean mussel, Mytilus galloprovincialis Lamark; and the Northern Bay Mussel, Mytilus trossulus Gould) during static 48-h exposures. These procedures will probably be useful for conducting static short-term chronic toxicity tests starting with embryos of other bivalve species (1)2 although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using procedures appropriate to a particular species or special needs and circumstances is more important than following prescribed procedures, results of tests conducted by using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained by using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting 48-h acute tests starting with embryos of bivalve molluscs.

1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications these procedures can be used to conduct acute tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments, and surface waters. Renewal tests might be preferable to static tests for materials that have a high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.

1.4 Results of toxicity tests with embryos of bivalve molluscs should usually be reported as the EC50 based on the total incompletely developed and dead organisms. It might also be desirable to report the LC50 based only on death. In some situations, it might only be necessary to determine whether a specific concentration is toxic to embryos or whether the EC50 is above or below a specific concentration.

1.5 This guide is arranged as follows:

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Keywords

acute toxicity ; bivalve mollusc larvae; bivalve molluscs embryos; blue mussel; Crassostrea gigas; Crassostrea virginica; hard clam; Mercenaria mercenaria; Mytilus edulis; Mytilus galloprovincialis; Mytilus spp.; oyster; short-term chronic toxicity;

ICS Code

ICS Number Code 07.100.20 (Microbiology of water)

DOI: 10.1520/E0724-21

Additional information

Species	10.1
Report	15
Test Material	9
Age	10.2
Test Chambers	7.3
Referenced Documents	2
Hazards	6
Source	8.2
Analytical Methods	12
Apparatus	7
Acceptability of Test	13
Brood Stock Source and Condition	10.4
Temperature	11.3
Significance and Use	5
Test Concentration(s)	9.3
Annex	Annex A1
Handling	10.3
Spawning and Fertilization	10.5
Quality	10.6
Summary of Guide	4
Duration of Test	11.6
Feeding	11.5
Calculation of Results	14
Acceptability	7.5
Facilities	7.1
Treatments	8.3
Terminology	3
Dilution Water	8
Stock Solution	9.2
Other Measurements	11.8
Biological Data	11.7
Construction Materials	7.2
Test Organisms	10
Experimental Design	11.1
Dissolved Oxygen	11.2
Beginning the Test	11.4
Cleaning	7.4
Characterization	8.4
Requirements	8.1
General	9.1
Procedure	11
ICS Codes	07.100.20 - Microbiology of water
	Section