{"id":608509,"date":"2024-11-05T22:42:15","date_gmt":"2024-11-05T22:42:15","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/iso-234182022\/"},"modified":"2024-11-05T22:42:15","modified_gmt":"2024-11-05T22:42:15","slug":"iso-234182022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/iso\/iso-234182022\/","title":{"rendered":"ISO 23418:2022"},"content":{"rendered":"<p>This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:<\/p>\n<p>a) handling of bacterial cultures;<\/p>\n<p>b) axenic genomic DNA isolation;<\/p>\n<p>c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;<\/p>\n<p>d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;<\/p>\n<p>e) metadata capture and sequence repository deposition;<\/p>\n<p>f) validation of the end-to-end WGS workflow (fit for purpose for intended application).<\/p>\n<p>This document is applicable to bacteria isolated from:<\/p>\n<p>\u2014\u00a0\u00a0\u00a0 products intended for human consumption;<\/p>\n<p>\u2014\u00a0\u00a0\u00a0 products intended for animal feed;<\/p>\n<p>\u2014\u00a0\u00a0\u00a0 environmental samples from food and feed handling and production areas;<\/p>\n<p>\u2014\u00a0\u00a0\u00a0 samples from the primary production stage.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><b>Microbiology of the food chain \u2014 Whole genome sequencing for typing and genomic characterization of bacteria \u2014 General requirements and guidance<\/b><\/p>\n<table style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/iso\/\"><b>ISO<\/b><\/a><\/td>\n<td>2022-06<\/td>\n<td>52<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":608524,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2634],"product_tag":[],"class_list":{"0":"post-608509","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-iso","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/608509","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/608524"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=608509"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=608509"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=608509"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}