{"id":340606,"date":"2024-10-19T23:51:57","date_gmt":"2024-10-19T23:51:57","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-iso-ts-131362012\/"},"modified":"2024-10-25T23:02:09","modified_gmt":"2024-10-25T23:02:09","slug":"bsi-pd-cen-iso-ts-131362012","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-iso-ts-131362012\/","title":{"rendered":"BSI PD CEN ISO\/TS 13136:2012"},"content":{"rendered":"

This Technical Specification describes the identification of Shiga toxin-producing Escherichia coli<\/i> (STEC) by means of the detection of the following genes:<\/p>\n

    \n
  1. \n

    the major virulence genes of STEC, stx<\/i> and eae<\/i> (References[<\/sup>2<\/sup>][<\/sup>3<\/sup>]<\/sup>);<\/p>\n<\/li>\n

  2. \n

    the genes associated with the serogroups O157, O111, O26, O103, and O145 (References[<\/sup>3<\/sup>][<\/sup>4<\/sup>]<\/sup>).<\/p>\n<\/li>\n<\/ol>\n

    In any case, when one or both of the stx<\/i> genes is\/are detected, the isolation of the strain is attempted.<\/p>\n

    The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS).<\/p>\n

    The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes.<\/p>\n

    This Technical Specification is applicable to:<\/p>\n

      \n
    1. \n

      products intended for human consumption and the feeding of animals;<\/p>\n<\/li>\n

    2. \n

      environmental samples in the area of food production and food handling;<\/p>\n<\/li>\n

    3. \n

      environmental samples in the area of primary production.<\/p>\n<\/li>\n<\/ol>\n

      PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
      PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
      7<\/td>\nIntroduction <\/td>\n<\/tr>\n
      8<\/td>\n1\tScope
      2\tNormative references <\/td>\n<\/tr>\n
      9<\/td>\n3\tTerms and definitions
      4\tPrinciple
      4.1\tGeneral
      4.2\tMicrobial enrichment <\/td>\n<\/tr>\n
      10<\/td>\n4.3\tNucleic acid extraction
      4.4\tTarget genes
      4.5\tDetection
      4.6\tIsolation
      5\tDiluents, culture media and reagents <\/td>\n<\/tr>\n
      11<\/td>\n5.1\tCulture media <\/td>\n<\/tr>\n
      12<\/td>\n5.2\tReagents for nucleic acid extraction
      5.3\tReagents for PCR
      6\tEquipment <\/td>\n<\/tr>\n
      13<\/td>\n7\tSampling
      8\tPreparation of test sample
      9\tProcedure
      9.1\tTest portion and initial suspension <\/td>\n<\/tr>\n
      14<\/td>\n9.2\tEnrichment
      9.3\tNucleic acid extraction
      9.4\tPCR amplification (for real-time PCR) <\/td>\n<\/tr>\n
      15<\/td>\n9.5\tStrain isolation
      10\tExpression of results
      11\tPerformance data <\/td>\n<\/tr>\n
      19<\/td>\nAnnex\u00a0A
      \n(normative)<\/p>\n

      Flow diagram of the screening procedure <\/td>\n<\/tr>\n

      20<\/td>\nAnnex\u00a0B
      \n(normative)<\/p>\n

      Flow diagram of the isolation and confirmation procedure <\/td>\n<\/tr>\n

      21<\/td>\nAnnex\u00a0C
      \n(informative)<\/p>\n

      Identification of Shiga toxin-producing Escherichia coli (STEC) by multiplex PCR amplification of virulence genes and detection of PCR products by agarose gel electrophoresis <\/td>\n<\/tr>\n

      25<\/td>\nAnnex\u00a0D
      \n(informative)<\/p>\n

      Internal amplification control <\/td>\n<\/tr>\n

      26<\/td>\nAnnex\u00a0E
      \n(informative)<\/p>\n

      Primers and probes for the PCR assays <\/td>\n<\/tr>\n

      28<\/td>\nAnnex\u00a0F
      \n(normative)<\/p>\n

      Isolation of STEC strains
      Annex\u00a0F
      \n(normative)<\/p>\n

      Isolation of STEC strains <\/td>\n<\/tr>\n

      29<\/td>\nBibliography
      Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

      Microbiology of food and animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups<\/b><\/p>\n\n\n\n\n
      Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
      BSI<\/b><\/a><\/td>\n2012<\/td>\n32<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":340613,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-340606","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/340606","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/340613"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=340606"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=340606"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=340606"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}